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Selective conjugated fatty acids inhibit guinea pig platelet aggregation.
Li, Guangming; Butz, Daniel; Dong, Baiyan; Park, Yeonhwa; Pariza, Michael W; Cook, Mark E.
Afiliación
  • Li G; Molecular and Environmental Toxicology, University of Wisconsin-Madison, 53706, USA.
Eur J Pharmacol ; 545(2-3): 93-9, 2006 Sep 18.
Article en En | MEDLINE | ID: mdl-16890934
Conjugated linoleic acids have been shown to reduce eicosanoid release from select tissues and/or cells. To elucidate effects of conjugated linoleic acid isomers on cyclooxygenase-1 (COX-1) activity and their application as platelet aggregation inhibitors, conjugated linoleic acid isomers and conjugated nonadecadienoic acid were incubated with ovine COX-1 and Raw264.7 macrophage to examine their effects on COX-1 activity. The effects were further examined in collagen and ADP-induced guinea pig whole blood platelet aggregation. Fatty acids tested were shown to inhibit COX-1 enzymatic activity. However, only 10t, 12c-conjugated linoleic acid, 9t, 11t-conjugated linoleic acid and conjugated nonadecadienoic acid inhibited collagen and ADP-induced platelet aggregation with IC(50) 125.9 microM (74.2 microM to 213.4 microM, 95% confidence interval), 99.3 microM (52.8 microM to 187.2 microM, 95% confidence interval) and 124.3 microM (85.1 microM to 181.5 microM, 95% confidence interval) respectively in collagen-induced aggregation. TxB(2) release was also appreciably inhibited by 10t, 12c-conjugated linoleic acid, 9t, 11t-conjugated linoleic acid and conjugated nonadecadienoic acid. Based on these data, we conclude 10t, 12c-conjugated linoleic acid, 9t, 11t-conjugated linoleic acid and conjugated nonadecadienoic acid are platelet aggregation inhibitors while 9c, 11t-conjugated linoleic acid is a moderate inhibitor and linoleic acid, and 9c, 11c-conjugated linoleic acid have no effect on whole blood platelet aggregation.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inhibidores de Agregación Plaquetaria / Agregación Plaquetaria / Ácidos Linoleicos Conjugados / Ácidos Grasos Límite: Animals Idioma: En Revista: Eur J Pharmacol Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inhibidores de Agregación Plaquetaria / Agregación Plaquetaria / Ácidos Linoleicos Conjugados / Ácidos Grasos Límite: Animals Idioma: En Revista: Eur J Pharmacol Año: 2006 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos