Effect of chaotropic mobile phase additives on retention behaviour of beta-blockers on various reversed-phase high-performance liquid chromatography columns.
J Chromatogr A
; 1133(1-2): 69-75, 2006 Nov 10.
Article
en En
| MEDLINE
| ID: mdl-16920124
ABSTRACT
In this study the effect of type and concentration of chaotropic counter anion in buffered mobile phase on retention behaviour of some beta-blockers was studied. Two types of anions (perchlorate, dihydrogen phosphate) were examined under different concentrations. Further different pH-values for each anion were used to investigate the effect of pH changes on chaotropic behaviour of these anions. The role of organic modifier (methanol, acetonitrile) type was also considered. All these parameters were studied for three different RP-columns (calixarene modified silica gel, monolithic RP-column and a conventional RP-column). The results indicate that all studied factors affected the chaotropic behaviour of the tested anions. Also the type of the used stationary phase has found to play an important role in retention behaviour of beta-blockers.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Antagonistas Adrenérgicos beta
/
Aniones
Idioma:
En
Revista:
J Chromatogr A
Año:
2006
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS