Enantioseparation by ligand-exchange using particle-loaded monoliths: capillary-LC versus capillary electrochromatography.
J Biochem Biophys Methods
; 70(1): 77-85, 2007 Feb 23.
Article
en En
| MEDLINE
| ID: mdl-16978701
ABSTRACT
Particle-loaded monoliths containing a polymethacrylamide backbone were prepared by suspending a silica-based chiral phase in the mixture of the monomers followed by in-situ polymerization in the capillary. As chiral selector l-4-hydroxyproline chemically bonded to 3 microm silica particles was used following the separation principle of ligand-exchange. Electrolytes containing Cu(II) ions were used. Amino acid enantiomers were separated by capillary-LC and CEC, whereby the latter showed the better resolution properties. For the chiral separation of alpha-hydroxy acids the EOF was reversed by copolymerizing diallyldimethylammonium chloride instead of vinylsulfonic acid as charge providing agent. Short columns of 6 cm were found to be sufficient in the case of CEC for baseline separations of amino acids with alpha values up to 5.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Liquida
/
Electrocromatografía Capilar
Idioma:
En
Revista:
J Biochem Biophys Methods
Año:
2007
Tipo del documento:
Article
País de afiliación:
Austria