Lateral magnocellular thalamic nucleus in rabbits: architecture and projections to cingulate cortex.

ABSTRACT

The lateral magnocellular nucleus (LM) contains the largest neurons in the rabbit thalamus, yet its cortical connections have not been described. This study evaluates the architecture, cingulate cortical connections, and spontaneous rate of neuronal discharges in LM. At its maximal mediolateral extent in coronal sections, LM underlies the laterodorsal and lateroposterior nuclei. It has a short medial and long lateral limb, both of which have high levels of cytochrome oxidase activity. On the basis of horseradish peroxidase and fluorescent dye injections, LM projects primarily to area 29 and posterior area 24. Projections to area 29d are topographically organized so that the medial limb of LM projects to rostral area 29d, mid levels of LM where the limbs join project to midlevels of area 29d and lateral parts of the lateral limb project to posterior area 29d. It is mainly the midportion of the lateral and medial limbs that projects to areas 29b and 29c. The anterior parts of these areas receive input from dorsal parts of LM, whereas posterior levels of these areas receive input from ventral LM. The midregion of LM also projects to caudal area 24. Injections of 3H-amino acids into area 29d anterogradely label neuronal processes in LM. Finally, single unit electrophysiological recordings from LM in halothane-anesthetized rabbits showed a unique pattern of spontaneous discharges. Over 70% of the LM neurons cycled through a number of different phases with a mean +/- S.E.M. peak discharge rate of 31 +/- 4.7 Hz. This high rate contrasts with the 17.6 +/- 3.2 Hz rate for neurons that maintained a constant rate of discharge and the 7.5 +/- 1.3 Hz rate of discharges for neurons in nuclei dorsal and ventral to LM. LM neurons are large, have high levels of cytochrome oxidase and spontaneous activity, and project extensively to the posterior cingulate cortex. These features suggest that LM neurons are highly active metabolically and may be fast conducting efferents to cingulate cortex.