Determination of interleukin-1alpha in human NCTC 2544 keratinocyte cells as a predictor of skin irritation from lysine-based surfactants.
Toxicol Lett
; 167(1): 40-6, 2006 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-16996234
Lysine derivative surfactants are a class of amino acid-based surfactants synthesized as lecithin analogues that deserve particular attention because of their low toxicity and high biocompatibility. To complete the toxicological profile of these surfactants, IL-1 alpha production (cell-associated and release to the culture medium) was determined as an in vitro method for predicting skin irritation. In addition, an MTT assay was used as a viability marker in keratinocytes NCTC 2544. Keratinocytes are a biologically relevant target for developing in vitro techniques to assess skin irritants: moreover, they are the principal source of the proinflammatory cytokine IL-1 alpha in the epidermis. Lysine derivatives proved to be less potent in stimulating IL-1 alpha synthesis and induced a lower release of this cytokine into the culture medium when compared to the anionic surfactant sodium dodecyl sulfate. Due to their low irritancy potential, lysine-based surfactants may offer promising applications in pharmaceutical and cosmetic preparations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tensoactivos
/
Queratinocitos
/
Interleucina-1alfa
/
Irritantes
/
Lisina
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Toxicol Lett
Año:
2006
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Países Bajos