Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions.
Proc Natl Acad Sci U S A
; 103(40): 14947-52, 2006 Oct 03.
Article
en En
| MEDLINE
| ID: mdl-17003132
The presence of the envelope glycoprotein Env in HIV-1 virions is essential for infectivity. To date, the molecular mechanism by which Env is packaged into virions has been largely unknown. Here, we show that TIP47 (tail-interacting protein of 47 kDa), which has been shown to interact with Env, also binds the MA (matrix) domain of HIV-1 Gag protein and that these three proteins form a ternary complex. Mutations in Gag that abrogate interaction with TIP47 inhibit Env incorporation and virion infectivity as well as colocalization between Gag and Env. We also show that TIP47 silencing impairs Env incorporation and infectivity and abolishes coimmunoprecipitation of Gag with Env. In contrast, overexpression of TIP47 increases Env packaging. Last, we demonstrate that TIP47 can interact simultaneously with Env and Gag. Taken together, our results show that TIP47 is a cellular cofactor that plays an essential role in Env incorporation, allowing the encounter and the physical association between HIV-1 Gag and Env proteins during the viral assembly process.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Gestacionales
/
Virión
/
Productos del Gen env
/
Productos del Gen gag
/
VIH-1
/
Péptidos y Proteínas de Señalización Intracelular
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2006
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos