Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis.
Folia Microbiol (Praha)
; 35(5): 437-42, 1990.
Article
en En
| MEDLINE
| ID: mdl-1702746
ABSTRACT
Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citometría de Flujo
/
Colorantes Fluorescentes
/
Mucorales
/
Micosis
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Folia Microbiol (Praha)
Año:
1990
Tipo del documento:
Article