Cytosolic and membrane-bound nitric oxide synthase.
Jpn J Pharmacol
; 56(2): 217-20, 1991 Jun.
Article
en En
| MEDLINE
| ID: mdl-1715413
ABSTRACT
Cytotoxic activated macrophages were sonicated and centrifuged. The activity of nitric oxide (NO) synthase was present in the supernatant and independent of Ca2+. The pellets were washed three times and treated with buffer containing 0.1% Triton X-100 or buffer alone, followed by centrifugation. The supernatant containing Triton X-100 showed NO synthase activity that was dependent on Ca2+, whereas the supernatant without the detergent had little activity. These data suggest that there are two forms of NO synthase cytosolic and membrane-bound enzymes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aminoácido Oxidorreductasas
Límite:
Animals
Idioma:
En
Revista:
Jpn J Pharmacol
Año:
1991
Tipo del documento:
Article
País de afiliación:
Japón