Therapeutic drug monitoring of tacrolimus with the dried blood spot method.
J Pharm Biomed Anal
; 44(3): 658-64, 2007 Jul 27.
Article
en En
| MEDLINE
| ID: mdl-17184953
ABSTRACT
In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tacrolimus
/
Monitoreo de Drogas
/
Inmunosupresores
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2007
Tipo del documento:
Article
País de afiliación:
Países Bajos