A novel microfluidic flow-injection analysis device with fluorescence detection for cation sensing. Application to potassium.
Anal Bioanal Chem
; 387(8): 2627-32, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17287929
ABSTRACT
A microfabricated device has been developed for fluorimetric detection of potassium ions without previous separation. It is based on use of a fluorescent molecular sensor, calix-bodipy, specially designed to be sensitive to and selective for the target ion. The device is essentially made of a Y-shape microchannel moulded in PDMS fixed on a glass substrate. A passive mixer is used for mixing the reactant and the analyte. The optical detection arrangement uses two optical fibres, one for excitation by a light-emitting diode, the other for collection of the fluorescence. This system enabled the flow-injection analysis of the concentration of potassium ions in aqueous solutions with a detection limit of 0.5 mmol L(-1) and without interference with sodium ions. A calibration plot was constructed using potassium standard solutions in the range 0-16 mmol L(-1), and was used for the determination of the potassium content of a pharmaceutical pill. Figure Photography of the microfluidic channel showing the ridges in the PDMS substrate at the top of the channel.
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Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY