Metabolism of 12(R)-hydroxyeicosatetraenoic acid by rat liver microsomes.
Biochim Biophys Acta
; 1123(1): 110-6, 1992 Jan 03.
Article
en En
| MEDLINE
| ID: mdl-1730042
ABSTRACT
The in vitro metabolism of 12(R)-hydroxyeicosatetraenoic acid was studied using freshly isolated rat liver microsomes. Ten metabolites were isolated and identified by a combination of ultraviolet spectroscopy and gas chromatography/mass spectrometry. The two major metabolites were dihydroxyeicosatetraenoic acids generated by omega/omega-1 hydroxylation. Oxidation at C-5 resulted in the formation of four leukotriene-like compounds, two of which differed from leukotriene B4 in double-bond geometry alone. The other two differed from leukotriene B4 in olefin geometry and C-5 configuration. Epoxidation at the 14,15-olefin resulted in the formation of two diastereomeric epoxy alcohols, while C-16 hydroxylation gave two diastereomeric dihydroxyeicosatetraenoic acids.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microsomas Hepáticos
/
Ácidos Hidroxieicosatetraenoicos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1992
Tipo del documento:
Article
País de afiliación:
Túnez