Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems.
Biotechnol Lett
; 29(5): 703-11, 2007 May.
Article
en En
| MEDLINE
| ID: mdl-17310326
ABSTRACT
Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for clinical production of recombinant proteins. The transfection with polyethylenimine (PEI) was shown here to be scalable using both stirred-tank bioreactors of 3- and 150-l and novel agitated cultivation vessels (50 ml ventilated centrifuge tubes and 1-l square-shaped glass bottles) that lack any instrumentation. The transient transfections spanned a range of working volumes from 2 ml to 80 l. The maximum transient recombinant antibody yield was 22 mg/l, the highest ever reported for a multiliter transfection in CHO. The transiently expressed protein had the same extent of glycosylation as the same antibody produced from a stably transfected recombinant CHO cell line.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Expresión Génica
/
Técnicas de Cultivo de Célula
/
Reactores Biológicos
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
Suiza