[Radioligand binding assay of progesterone receptors on normal fertile human sperm membrane].
Zhonghua Nan Ke Xue
; 13(2): 114-7, 2007 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-17345764
ABSTRACT
OBJECTIVE:
To investigate the progesterone-binding site on the normal fertile human sperm membrane after 2 hours of in vitro capacitation.METHODS:
Viable spermatozoa were selected by a swim-up method. After 2 hours of in vitro capacitation, multipoint saturation binding experiments were performed. Sperm suspension and increasing concentrations of progesterone-11alpha-glucuronide-[125I] iodotyramine (125I-P) were added to 7 total binding tubes respectively, and equal amounts of sperm suspension and 125I-P were added to another 7 corresponding non-specific binding tubes in the presence of 10 micromol/L progesterone. After incubation for 1 hour at 4 degrees C, the radioactivity of both the tubes and the pellets after centrifugation was measured respectively. The equilibrium dissociation constant (Kd) and maximum binding capacity (Bmax) were calculated using the mathematical model of single site multi-point saturation method of Scatchard function and least-squares regression.RESULTS:
Kd was (0.61 +/- 0.04) nmol/L and Bmax was (830 +/- 344) sites/cell. The significance test of the regression equation indicated that r = -0.980, P < 0.01.CONCLUSION:
There is a high affinity and low capacity binding site for the progesterone (progesterone receptor) on the normal fertile human sperm membrane.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Receptores de Progesterona
Límite:
Adult
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China