NK-KIR ligand identification: a quick Q-PCR approach for HLA-C epitope typing.
Tissue Antigens
; 69(4): 334-7, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17389017
ABSTRACT
Interaction of donor natural killer (NK)-cell-associated killer cell immunoglobulin-like receptors (KIRs) with the patient's human leukocyte antigen-C (HLA-C) ligands can result in an alloreactive NK response after haematopoietic stem cell transplantation. In many retrospective studies, additional HLA-C-typing data are required to predict NK-cell alloreactivity. We developed a Taqman assay using the quantitative polymerase chain reaction (Q-PCR) technique that facilitates HLA-C epitope typing, allowing the assignment of HLA-C group 1 or 2 alleles based on the dimorphism at residues 77 and 80 rather than based on the sequence specific priming (SSP) and sequence-based typing allele types. Q-PCR analysis for HLA-C epitope detection showed three clusters reflecting homozygous group 1 or 2 and heterozygous samples. This new approach introduces a quick HLA-C epitope screening method to define the presence of the ligand for the KIR-HLA-C interaction.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Prueba de Histocompatibilidad
/
Células Asesinas Naturales
/
Antígenos HLA-C
/
Reacción en Cadena de la Polimerasa
/
Epítopos
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Tissue Antigens
Año:
2007
Tipo del documento:
Article
País de afiliación:
Países Bajos