Modular mass spectrometric tool for analysis of composition and phosphorylation of protein complexes.
PLoS One
; 2(4): e358, 2007 Apr 04.
Article
en En
| MEDLINE
| ID: mdl-17406682
ABSTRACT
The combination of high accuracy, sensitivity and speed of single and multiple-stage mass spectrometric analyses enables the collection of comprehensive sets of data containing detailed information about complex biological samples. To achieve these properties, we combined two high-performance matrix-assisted laser desorption ionization mass analyzers in one modular mass spectrometric tool, and applied this tool for dissecting the composition and post-translational modifications of protein complexes. As an example of this approach, we here present studies of the Saccharomyces cerevisiae anaphase-promoting complexes (APC) and elucidation of phosphorylation sites on its components. In general, the modular concept we describe could be useful for assembling mass spectrometers operating with both matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) ion sources into powerful mass spectrometric tools for the comprehensive analysis of complex biological samples.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Espectrometría de Masa por Ionización de Electrospray
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos