Cyclosporin A potentiates the cytotoxic effects of methyl methanesulphonate in HL-60 and K562 cells.
Altern Lab Anim
; 35(1): 79-85, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17411355
Methyl methanesulphonate (MMS) is a DNA damaging agent, which induces oxidative stress, ATP depletion, and consequently, cell death, in HL-60 and K562 cells. The cell death induced by MMS predominantly exhibited the morphological and biochemical hallmarks of necrosis. A minor population of dying cells exhibited apoptotic hallmarks, especially in K562 cell cultures. Cyclosporin A (CsA) was used to modulate the MMS-induced cell death. Our results indicated that CsA did not prevent cells from dying, but changed the mode of death from necrotic to apoptotic. Surprisingly, CsA enhanced oxidative stress and increased the overall number of dead cells. Based on these results, we conclude that the modulatory effect of CsA on MMS-induced cell death might arise from an interference by CsA with mitochondrial metabolism, rather than from inhibition of the MMS efflux mediated by P-glycoprotein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ciclosporina
/
Células HL-60
/
Células K562
/
Inmunosupresores
/
Metilmetanosulfonato
Límite:
Humans
Idioma:
En
Revista:
Altern Lab Anim
Año:
2007
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
Reino Unido