Long terminal repeat sequences from virulent and attenuated equine infectious anemia virus demonstrate distinct promoter activities.
Virus Res
; 128(1-2): 58-64, 2007 Sep.
Article
en En
| MEDLINE
| ID: mdl-17499380
ABSTRACT
In the early 1970s, the Chinese Equine Infectious Anemia Virus (EIAV) vaccine, EIAV(DLA), was developed through successive passages of a wild-type virulent virus (EIAV(L)) in donkeys in vivo and then in donkey macrophages in vitro. EIAV attenuation and cell tropism adaptation are associated with changes in both envelope and long terminal repeat (LTR). However, specific LTR changes during Chinese EIAV attenuation have not been demonstrated. In this study, we compared LTR sequences from both virulent and attenuated EIAV strains and documented the diversities of LTR sequence from in vivo and in vitro infections. We found that EIAV LTRs of virulent strains were homologous, while EIAV vaccine have variable LTRs. Interestingly, experimental inoculation of EIAV(DLA) into a horse resulted in a restriction of the LTR variation. Furthermore, LTRs from EIAV(DLA) showed higher Tat transactivated activity than LTRs from virulent strains. By using chimeric clones of wild-type LTR and vaccine LTR, the main difference of activity was mapped to the changes of R region, rather than U3 region.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Variación Genética
/
Monocitos
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Regiones Promotoras Genéticas
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Virus de la Anemia Infecciosa Equina
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Secuencias Repetidas Terminales
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Caballos
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Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Virus Res
Asunto de la revista:
VIROLOGIA
Año:
2007
Tipo del documento:
Article