Rapid identification of an HLA-B*1501-restricted vaccinia peptide antigen.
Vaccine
; 25(24): 4715-22, 2007 Jun 11.
Article
en En
| MEDLINE
| ID: mdl-17499403
ABSTRACT
We identified a modified vaccinia virus Ankara (MVA)-peptide recognized by T cells of a vaccinated patient, applying a modified expression-based procedure. CD8(+) T cells reactive with MVA were expanded from peripheral blood of a patient after MVA-vaccination. The restricting HLA-elements and the antigen were determined applying stably HLA-class I-transfected K562 cells that were either infected wit MVA or transfected with a panel of MVA open reading frames (ORF). The T cells recognized the ORF 28R (K7R) gene product in association with HLA-B*1501 and the peptide-coding region was localized applying truncated in vitro transcribed mRNA. The 9-mer peptide encoded (ORF 28(25-33), SIIDLIDEY) was identified that was also recognized by ex vivo CD8(+) T cells from the patient and further healthy individuals.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Vaccinia
/
Antígenos HLA-B
/
Epítopos de Linfocito T
/
Antígenos Virales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Vaccine
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania