Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5.
Biotechnol Lett
; 29(8): 1227-32, 2007 Aug.
Article
en En
| MEDLINE
| ID: mdl-17505784
ABSTRACT
Fusarium venenatum A3/5 was transformed using the Aspergillus niger expression plasmid, pIGF, in which the coding sequence for the F. solani f. sp. pisi cutinase gene had been inserted in frame, with a KEX2 cleavage site, with the truncated A. niger glucoamylase gene under control of the A. niger glucoamylase promoter. The transformant produced up to 21 U cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. Glucoamylase (165 U l(-1) or 8 mg l(-1)) was also produced. Both the transformant and the parent strain produced cutinase in medium containing cutin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Fusarium
Idioma:
En
Revista:
Biotechnol Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
Dinamarca