Tumor necrosis factor alters cytoskeletal organization and barrier function of endothelial cells.
Int Arch Allergy Appl Immunol
; 96(1): 84-91, 1991.
Article
en En
| MEDLINE
| ID: mdl-1752699
ABSTRACT
Treatment of human umbilical cord vein endothelial cells with tumor necrosis factor results in marked changes in cell shape and cytoskeletal organization. After 4 h of treatment, these cells loose reciprocal contacts with the formation of intercellular gaps. This retraction reaches a maximum after 6 h when most stress fibers staining for F-actin disappear and vinculin becomes diffused in the cytoplasm. Such changes spontaneously reverse after 24 h in the presence of tumor necrosis factor or after 2 h of incubation in fresh medium. After treatment with tumor necrosis factor, endothelial monolayers become permeable to albumin because of gaps that form between cells. Normal human serum, plasma alpha 1-proteinase inhibitor and an anti-inflammatory peptide that decrease synthesis of platelet-activating factor inhibit the changes induced by tumor necrosis factor. Furthermore, receptor antagonists of platelet-activating factor have the same effect. These findings suggest that platelet-activating factor is a secondary mediator responsible for the changes in cell shape and cytoskeletal organization, and for the leakiness of endothelial monolayers.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endotelio Vascular
/
Factor de Necrosis Tumoral alfa
/
Péptidos Catiónicos Antimicrobianos
Límite:
Humans
Idioma:
En
Revista:
Int Arch Allergy Appl Immunol
Año:
1991
Tipo del documento:
Article
País de afiliación:
Italia