[Effects of antisense epidermal growth factor receptor oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 29(2): 213-6, 2007 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-17536271
ABSTRACT
OBJECTIVE:
To explore the effects of antisense epidermal growth factor receptor (EGF-R) oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.METHODS:
c-jun DNA binding activity after ultraviolet-B (UVB) irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.RESULTS:
Compared with control groups, c-jun activity increased significantly in UVB (10, 20, 30 mJ/cm2) irradiation groups (P < 0.05). EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 microg/ml concentrations (P < 0.01).CONCLUSION:
The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides, which is transfected to keratinocytes and mediated by lipofectamine.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Queratinocitos
/
Oligodesoxirribonucleótidos Antisentido
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Receptores ErbB
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Año:
2007
Tipo del documento:
Article
País de afiliación:
China