Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.
Biotechnol Lett
; 29(12): 2001-5, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17628748
Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Ácido Salicílico
/
Ciclopentanos
/
Eschscholzia
/
Alcaloides
/
Benzofenantridinas
/
Oxilipinas
/
Acetatos
Idioma:
En
Revista:
Biotechnol Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
Corea del Sur
Pais de publicación:
Países Bajos