[One step time-resolved fluorescence immunoassay for direct estimation of microcystins].
Huan Jing Ke Xue
; 28(4): 872-5, 2007 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-17639952
ABSTRACT
A direct-competitive time-resolved fluorescence immunoassay (TRFIA) for microcystin detection was established, which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG. The optimal dilution of europium labeled MCLR-BSA conjugant is 1/50 and most appropriate titration of anti-microcystin-LR (MCLR) monoclonal antibodies is 100 ng/mL. The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05 ng/mL to 10 ng/mL. The detection limit of the method is 0.02 ng/mL for MCLR, recoveries for microcystin (> 94%) in quantitative range is satisfactory.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fluoroinmunoensayo
/
Microcistinas
Idioma:
Zh
Revista:
Huan Jing Ke Xue
Año:
2007
Tipo del documento:
Article
País de afiliación:
China