CUBIC: a three-dimensional colored projection of Consort 30 generated trivariate flow cytometric data.
Cytometry
; 12(6): 570-8, 1991.
Article
en En
| MEDLINE
| ID: mdl-1764980
ABSTRACT
The CUBIC program displays three-dimensional colored dot plots of flow cytometric trivariate data collected by unmodified commercial instruments (FACScan flow cytometer, FACS 440 cell sorter). Assuming a bimodal distribution of the fluorescence intensity of the cells, the eight theoretical subpopulations involved in a three-color fluorescence histogram are clearly localized in the 3-D space by colored dots that are clustered near each corner of a cubic frame. Rotation, tilting, and zoom functions are available. Table look-up is not needed. CUBIC was illustrated by two experiments 1) three-color immunofluorescence of antigens on human lymphocytes using monoclonal antibodies conjugated either to fluorescein (FITC), to R-phycoerythrin (PE), or to biotin revealed by a streptavidin coupled to a PE-Texas red tandem conjugate (TC); 2) two-color immunofluorescence of CD4 and CD8 antigens on thymocytes of healthy or preleukemic mice correlated to the DNA content quantified by 7-amino-actinomycin D (7-AAD). The three fluorescences were excited by a single argon-ion laser emitting at 488 nm.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Gráficos por Computador
/
Programas Informáticos
/
Separación Celular
/
Técnica del Anticuerpo Fluorescente
/
Citometría de Flujo
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cytometry
Año:
1991
Tipo del documento:
Article
País de afiliación:
Bélgica