Variable actin dynamics requirement for the exit of different cargo from the trans-Golgi network.
FEBS Lett
; 581(20): 3875-81, 2007 Aug 07.
Article
en En
| MEDLINE
| ID: mdl-17651738
ABSTRACT
Efficient post-Golgi trafficking depends on microtubules, but actin filaments and actin-associated proteins are also postulated. Here we examined, by inverse fluorescence recovery after photobleaching, the role of actin dynamics in the exit from the TGN of fluorescent-tagged apical or basolateral and raft or non-raft-associated cargoes. Either the actin-stabilizing jasplakinolide or the actin-depolymerising latrunculin B variably but significantly inhibited post-Golgi traffic of non-raft associated apical p75NTR and basolateral VSV-G cargoes. The TGN-exit of the apical-destined VSV-G mutant was impaired only by latrunculin B. Strikingly, the raft-associated GPI-anchor protein was not affected by either actin toxin. Results indicate that actin dynamics participates in the TGN egress of both apical- and basolateral-targeted proteins but is not needed for apical raft-associated cargo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Actinas
/
Red trans-Golgi
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
España