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High-performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill.
Rodrigues, Clenilson M; Rinaldo, Daniel; Sannomiya, Miriam; dos Santos, Lourdes C; Montoro, Paola; Piacente, Sonia; Pizza, Cosimo; Vilegas, Wagner.
Afiliación
  • Rodrigues CM; UNESP, São Paulo State University, Institute of Chemistry. Organic Chemistry Department, CP 355, CEP 14800-900, Araraquara, São Paulo, Brazil.
Phytochem Anal ; 19(1): 17-24, 2008.
Article en En | MEDLINE | ID: mdl-17654510
The isolation of polyphenolic compounds from an infusion of the Brazilian plant Davilla elliptica (Dilleniaceae), used as tea by virtue of its digestive properties, is described. An improved preparative HPLC method was used in order to isolate pure polyphenols from the complex mixture. Liquid-liquid extraction and solid-phase extraction were employed to minimise the interference of polymeric compounds and to provide an enriched fraction of the compounds of interest. The identification of the isolated compounds was performed using analytical HPLC as well as direct injection electrospray ionisation ion trap tandem mass spectrometry (ESI-IT-MS/MS). The high flavonoid content suggests that D. elliptica may be a promising source of compounds to produce natural phytomedicines.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Flavonoides / Cromatografía Líquida de Alta Presión / Dilleniaceae Tipo de estudio: Diagnostic_studies Idioma: En Revista: Phytochem Anal Asunto de la revista: BOTANICA / QUIMICA Año: 2008 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Flavonoides / Cromatografía Líquida de Alta Presión / Dilleniaceae Tipo de estudio: Diagnostic_studies Idioma: En Revista: Phytochem Anal Asunto de la revista: BOTANICA / QUIMICA Año: 2008 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido