[Expression of recombinant actin 5C from Drosophila in the methylotrophic yeast Pichia pastoris].
Tsitologiia
; 49(4): 300-10, 2007.
Article
en Ru
| MEDLINE
| ID: mdl-17657944
ABSTRACT
A system for actin expression in cells of yeast Pichia pastoris was constructed. Drosophila actin 5C, by 90% homologous to beta-actin of higher eukaryotes, was used as a target protein. To improve the procedures of target protein biosynthesis in yeast cells and of extraction and purification of recombinant actin the fusion protein GFP-actin 5C, having fluorescence protein GFP as a reporter part, was expressed and purified. The dimensions and resistance of yeast cells producing recombinant actin were characterized. It was shown that the size and form of cells depended on the accumulation of recombinant protein. The purified fusion protein was used for obtaining polyclonal antibody for testing recombinant actin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
/
Ingeniería de Proteínas
/
Actinas
/
Drosophila
Límite:
Animals
Idioma:
Ru
Revista:
Tsitologiia
Año:
2007
Tipo del documento:
Article