Fabrication and characterization of chitosan microcarrier for hepatocyte culture.
J Mater Sci Mater Med
; 18(11): 2211-4, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-17665121
ABSTRACT
Using chitosan as raw materials, a suitable size (300-500 mum) of porous microcarrier was fabricated by suspension crosslinking and lyophilizing method, which made the carrier has an average pore size of 50 microm and 86% porosity. The microcarrier was modified with lactose and maltose respectively. Various factors that influenced the preparation of microcarrier were studied and the reaction conditions were optimized. Rat hepatocytes cultured on modified microcarrier retained a spherical shape which is similar to those in vivo and formed aggregates. The metabolic activities of cells on lactose-modified were higher than those on maltose-modified microcarrier. The highest albumin secretion reached 54.8 microg/10(6 )cells/d, and the highest urea synthesis reached 4.65 micromol/10(6)cells/d, which may be promoted by the formation of cellular aggregates. In conclusion, lactose-modified porous microcarrier is promising scaffold for hepatocytes culture.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Portadores de Fármacos
/
Hepatocitos
/
Quitosano
/
Microesferas
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Mater Sci Mater Med
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China