[Surface-enhanced Raman spectra of oxidation damnification of fetal bovine serum by ozone].
Guang Pu Xue Yu Guang Pu Fen Xi
; 27(6): 1140-2, 2007 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-17763777
ABSTRACT
Fetal bovine serum was treated by ozone for 1 hour and 3 hours before getting its surface-enhanced Raman spectra from 200 to 1 800 cm(-1). Treated with ozone for 1 hour, it shows a significant decrease in band intensity. Treated with ozone for 3 hours, the band intensity has a further decrease but not so obviously, which means that oxidation of ozone is short lived. Treated with ozone, the orderly conformations of main chains in protein such as alpha-helix, beta-sheet and beta-corner are damaged seriously. Aromatic side chains and C-S of Cys and Met also are damnified greatly. All this means that strong oxidation of ozone results in denaturation, conformational changes and even degradation in protein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ozono
/
Espectrometría Raman
/
Sangre Fetal
Límite:
Animals
Idioma:
Zh
Revista:
Guang Pu Xue Yu Guang Pu Fen Xi
Año:
2007
Tipo del documento:
Article
País de afiliación:
China