Development and validation of a liquid chromatographic method for the determination of branched-chain amino acids in new dosage forms.
J Pharm Biomed Anal
; 45(3): 456-64, 2007 Nov 05.
Article
en En
| MEDLINE
| ID: mdl-17875379
ABSTRACT
A reversed-phase liquid chromatographic method (RP-LC) is proposed and validated for the analysis of branched-chain amino acids (l-leucine, l-isoleucine and l-valine) in new pharmaceutical formulations. The pre-column derivatization reaction of these amino acids with 2,4-dinitrofluorobenzene (DNFB) has been investigated considering the matrix effect. The compound reacts at 60 degrees C for 10 min at pH 9 with the amino function, in presence of cetyltrimethylammonium bromide (CTAB), to give adducts that have been separated on a RP amide C16 column and detected at lambda=360 nm. Linear responses were observed for each derivative. The intra-day precision (R.S.D.) was
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Preparaciones Farmacéuticas
/
Aminoácidos de Cadena Ramificada
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2007
Tipo del documento:
Article
País de afiliación:
Italia