Side population cells expressing ABCG2 in human adult dental pulp tissue.
Int Endod J
; 40(12): 949-58, 2007 Dec.
Article
en En
| MEDLINE
| ID: mdl-17916067
ABSTRACT
AIM:
To investigate the presence of side population (SP) cells by the Hoechst exclusion method in human adult dental pulp tissue.METHODOLOGY:
Human adult dental pulp-derived cells were generated from third molar teeth. The cells were stained with Hoechst 33342 and sorted into SP cells or non-SP cells [main population (MP) cells]. Both cell types were compared with cell growth and RT-PCR analyses.RESULTS:
SP cells that express ABCG2, Nestin, Notch-1 and alpha-smooth muscle actin were found at frequencies ranging from 0.67% to 1.02%. This SP profile disappeared in the presence of verapamil. These SP cells expressed dentine sialophosphoprotein and dentine matrix protein-1 when cultured in osteogenic medium.CONCLUSION:
Human adult dental pulp tissue contains SP cells that differentiate into odontoblast-like cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Transportadoras de Casetes de Unión a ATP
/
Pulpa Dental
/
Proteínas de Neoplasias
/
Odontoblastos
Límite:
Adolescent
/
Adult
/
Humans
Idioma:
En
Revista:
Int Endod J
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón