PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution.
Mar Biotechnol (NY)
; 10(2): 122-7, 2008.
Article
en En
| MEDLINE
| ID: mdl-17922226
ABSTRACT
A simple and effective protocol is described for multiplex polymerase chain reaction (PCR) amplification of single cells of Karenia brevis. The protocol requires minimum processing, avoids additions that might dilute target DNA template, and can be used on cells preserved in Lugol's iodine preservative. Destaining of Lugol's-preserved cells with sodium thiosulfate allowed successful amplification of single-copy, nuclear-encoded microsatellites in single cells of K. brevis that have been preserved for up to 6 years.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dinoflagelados
/
Reacción en Cadena de la Polimerasa
/
Repeticiones de Microsatélite
Límite:
Animals
Idioma:
En
Revista:
Mar Biotechnol (NY)
Asunto de la revista:
BIOLOGIA
/
BIOTECNOLOGIA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos