Your browser doesn't support javascript.
loading
New host nuclear functions are not required for the modifications of the parasitophorous vacuole of Toxoplasma.
Romano, Julia D; Bano, Nazneen; Coppens, Isabelle.
Afiliación
  • Romano JD; Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205, USA.
Cell Microbiol ; 10(2): 465-76, 2008 Feb.
Article en En | MEDLINE | ID: mdl-17970763
ABSTRACT
The obligate intracellular parasite Toxoplasma develops within a parasitophorous vacuole (PV) uniquely adapted for its survival in mammalian cells. Post-invasion events extensively modify the PV, resulting in interactions with host cell structures. Recent studies emphasized that Toxoplasma is able to co-opt host gene expression, suggesting that host transcriptional activities are required for parasite infection. By using an experimental enucleation model, we investigated the potential need for Toxoplasma to modify its PV by modulating gene expression in the cell wherein it resides. Unexpectedly, cytoplasts can be actively invaded by Toxoplasma and sustain its replication inside a vacuole until egress and transmission to neighbouring cells. Although randomly distributed in the cytoplast, the PV associates with host centrosomes and the Golgi, is surrounded by host microtubules, and recruits host endoplasmic reticulum and mitochondria. Parasites are proficient in diverting exogenous nutrients from the endocytic network of cytoplasts. In enucleated cells invaded by an avirulent strain of T. gondii, the PV can normally transform into cysts. These observations suggest that new host nuclear functions are not proximately required for the post-invasion events underlying the remodelling of the host cell in which the parasites are confined, and therefore for the generation of infectious parasites in vitro.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Vacuolas / Núcleo Celular Límite: Animals / Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Vacuolas / Núcleo Celular Límite: Animals / Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos