Chemical specificity in three-dimensional imaging with multiplex coherent anti-Stokes Raman scattering microscopy.

ABSTRACT

We demonstrate the three-dimensional (3D) imaging capabilities and chemical specificity of multiplex coherent anti-Stokes Raman scattering microscopy. The simultaneous acquisition of a significant part of the vibrational spectrum at each specimen position permits straightforward differentiation among chemical species. 3D imaging is illustrated with a lipid multilamellar vesicle, and lateral and axial resolutions are determined.