Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry.
J Chromatogr A
; 1189(1-2): 398-405, 2008 May 02.
Article
en En
| MEDLINE
| ID: mdl-18035361
ABSTRACT
The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography-ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hordeum
/
Flavonoides
/
Grano Comestible
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
2008
Tipo del documento:
Article
País de afiliación:
República Checa