Proteomic investigation of the aggregation phenomenon in Lactobacillus crispatus.
Biochim Biophys Acta
; 1784(2): 335-42, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-18078834
ABSTRACT
Aggregation process affects the ability of Lactobacillus crispatus, a probiotic, to survive into the gastro-intestinal environment and to adhere to the intestinal mucosa. To elucidate mechanisms underlying this process, a comparative proteomic study was carried out on a wild type strain M247 and its spontaneous isogenic mutant Mu5, which had lost the aggregative phenotype. Results highlighted an overall lower amount of enzymes involved in carbohydrate transport and metabolism in strain M247 compared to strain Mu5, suggesting a reduction in the general growth rate, probably caused by nutrient limitation in cell aggregates, coherently with the phenotypic traits of the strains. Moreover, the up-regulation of a putative elongation factor Tu in the wild type M247 strain could suggest a role of this particular protein in the adhesion mechanism of L. crispatus.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Lactobacillus
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2008
Tipo del documento:
Article
País de afiliación:
Italia