Application of fluorescence in situ hybridization technique to detect simazine-degrading bacteria in soil samples.
Chemosphere
; 71(4): 703-10, 2008 Mar.
Article
en En
| MEDLINE
| ID: mdl-18082866
ABSTRACT
We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi and Henares) with a history of simazine treatments, and two natural soils (Soto and Monza), without previous exposure to simazine, were studied. AtzB1 probe-target cells were found only in the agricultural soils and, in a greater percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest percentage of AtzB1 probe-target cells in Lodi was accompanied by a greater mineralization rate, compared to the Henares soil. The FISH method used in this study was suitable for the detection of simazine-degrading bacteria and could be a useful indicator of the potential of soil bioremediation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Simazina
/
Microbiología del Suelo
/
Bacterias
/
Biodegradación Ambiental
/
Hibridación Fluorescente in Situ
Tipo de estudio:
Guideline
Idioma:
En
Revista:
Chemosphere
Año:
2008
Tipo del documento:
Article
País de afiliación:
España