Determination of 9-[(2-phosphonylmethoxy)ethyl]adenine in rat urine by high-performance liquid chromatography with fluorescence detection.

Russell, J W; Marrero, D; Whiterock, V J; Klunk, L J; Starrett, J E

Russell, J W; Marrero, D; Whiterock, V J; Klunk, L J; Starrett, J E.

Afiliación

Russell JW; Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Company, Wallingford, CT 06492.

J Chromatogr ; 572(1-2): 321-6, 1991 Dec 06.

Article en En | MEDLINE | ID: mdl-1818068

ABSTRACT

ABSTRACT

A high-performance liquid chromatographic (HPLC) method for the determination of 9-[(2-phosphonylmethoxy)ethyl]adenine (PMEA) in urine is described. The procedure includes treatment of the urine sample with chloroacetaldehyde to form the fluorescent 1,N6-ethenoadenosine derivative, which was analyzed by reversed-phase HPLC with fluorometric detection. Validation of the method showed good sensitivity, precision and reproducibility. The method is useful for the study of urinary excretion of PMEA in the rat.

Asunto(s)

Adenina/análogos & derivados; Antivirales/orina; Organofosfonatos; Adenina/orina; Animales; Cromatografía Líquida de Alta Presión; Masculino; Ratas; Ratas Endogámicas; Reproducibilidad de los Resultados; Espectrometría de Fluorescencia

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Adenina / Organofosfonatos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Chromatogr Año: 1991 Tipo del documento: Article

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