Influence of the stationary phase on the stability of thalidomide and comparison of different methods for the quantification of thalidomide in tablets using high-temperature liquid chromatography.
J Pharm Biomed Anal
; 46(4): 625-30, 2008 Mar 13.
Article
en En
| MEDLINE
| ID: mdl-18248931
ABSTRACT
In this paper, three different HPLC methods for the quantification of thalidomide in tablets were developed and compared. The comparison of a conventional method at 30 degrees C with two high-temperature methods at 180 degrees C showed equal results. Using high-temperature HPLC (HT-HPLC), faster analysis times could be achieved. We have also focused on analyte stability and could show that the stationary phase has a pronounced effect on the on-column degradation of thalidomide at high temperatures. Virtually no degradation occurs if a polystyrene divinylbenzene column is used, whereas thalidomide is completely degraded at 180 degrees C when a carbon clad zirconium dioxide column is used.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Talidomida
/
Cromatografía Líquida de Alta Presión
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2008
Tipo del documento:
Article
País de afiliación:
Alemania