Effect of interleukins and anti-CD3 monoclonal antibody on the proliferation of thymocytes from irradiated mice.
Thymus
; 17(2): 97-113, 1991 Mar.
Article
en En
| MEDLINE
| ID: mdl-1827549
ABSTRACT
The responsiveness of thymocytes on day 8 after irradiation to mitogens or anti-CD3 monoclonal antibody was evaluated in the presence of interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 6 (IL-6) or phorbol-myristate-acetate (PMA). After irradiation, the thymocytes were poorly responsive to T cell mitogens (Concanavalin A, phytohemagglutinin) and the defect could not be overcome by exogenous IL-2, IL-4, IL-6 or by PMA. In contrast, the combination of the calcium ionophore (A23187) and PMA stimulated thymocyte proliferation to a normal level. The anti-CD3 antibody associated with PMA activated thymocytes above the control values, but this was not observed when anti-CD3 was associated with either IL-2 or IL-4. These results suggest that in the thymic populations present early after irradiation 1) the weak proliferative response to mitogens could be related to a defect at a thymocyte level associated or not to an accessory cell deficiency, 2) the intracellular mechanisms involved in T cell proliferation were not altered, 3) the T cell antigen-receptor/CD3 complex was functional.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T
Límite:
Animals
Idioma:
En
Revista:
Thymus
Año:
1991
Tipo del documento:
Article
País de afiliación:
Francia