Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network.
J Cell Sci
; 121(Pt 6): 843-53, 2008 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-18303049
ABSTRACT
The protein kinase Cdc2p is the master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. It is required both for entry into mitosis and for onset of DNA replication. Cdc2p must be inactivated to permit exit from mitosis, licensing of replication origins and cytokinesis. To study the role of Cdc2p in greater detail, we generated a cdc2 allele that is sensitive to an inhibitory ATP analogue. We show that the inhibitor-induced cell cycle arrest is reversible and examine the effect of inhibiting Cdc2p on the regulation of the septation initiation network (SIN), which controls the initiation of cytokinesis in S. pombe. We found that specific inactivation of Cdc2p in a mitotically arrested cell promotes the asymmetrical recruitment of SIN proteins to the spindle poles and the recruitment of the most downstream SIN components and beta-(1,3) glucan synthase to the contractile ring. Thus, we conclude that inactivation of Cdc2p is sufficient to activate the SIN and promote cytokinesis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
Proteína Quinasa CDC2
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Proteínas de Schizosaccharomyces pombe
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Citocinesis
Idioma:
En
Revista:
J Cell Sci
Año:
2008
Tipo del documento:
Article
País de afiliación:
Suiza