Magnesium, manganese, and divalent cation transport assays in intact cells.
Methods Mol Biol
; 394: 289-305, 2007.
Article
en En
| MEDLINE
| ID: mdl-18363241
ABSTRACT
Protocols are described for the measurement of radioisotopic cation influx and efflux in bacteria using Salmonella as a model system. Methods are discussed for both the use of primary radioisotopes for measurement, e.g., using 54Mn2+ to measure Mn2+ influx, and the use of surrogate radioisotopes when the primary radioisotope is not available, e.g., use of 63Ni2+ or 57Co2+ in place of 28Mg2+ to measure Mg2+ influx. Both vacuum filtration and centrifugation assays are described. In addition, the use and misuse of chelating agents is discussed.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmonella
/
Magnesio
/
Manganeso
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos