[Synthesis, secretion, and proteolytic degradation of diphtheria toxin in Escherichia coli]. / Sintez, sekretsiia i proteoliticheskaia degradatsiia difteriinogo toksina v Escherichia coli.
Mol Gen Mikrobiol Virusol
; (10): 3-8, 1991 Oct.
Article
en Ru
| MEDLINE
| ID: mdl-1836836
ABSTRACT
The recombinant plasmids have been constructed encoding the synthesis of a full-sized diphtheria toxin from its own or PR, PL-promoters of bacteriophage lambda in Escherichia coli cells. The high level constitutive synthesis of toxin results in slow cell growth and plasmid elimination. The toxin was mainly detected in the periplasm, partially in the membrane and to a less extent in the cytoplasm and culturing medium. The dimeric form of toxin was found in the cytoplasm. Participation of toxin B-subunit in secreting of the toxin into culturing medium is discussed. Proteolytic degradation of the synthesized toxin in different Escherichia coli strains was demonstrated. The process takes place in cytoplasm and periplasm mainly. The main enzyme participating in the process is a La-protease. The data on proteolysis obtained by immunoprecipitation immunoblotting, affinity chromatography and in mini-cells of Escherichia coli are summarized.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Toxina Diftérica
/
Escherichia coli
Idioma:
Ru
Revista:
Mol Gen Mikrobiol Virusol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
/
VIROLOGIA
Año:
1991
Tipo del documento:
Article