An improved PCR method for gender identification of eagles.
Mol Cell Probes
; 22(3): 184-8, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18385011
ABSTRACT
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Águilas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Aspecto:
Determinantes_sociais_saude
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Probes
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Taiwán