[Epidemiological survey on the infection of hepatitis E virus among pigs in Henan province].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 22(1): 24-6, 2008 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-18414692
ABSTRACT
OBJECTIVE:
To investigate hepatitis E virus (HEV) infection among pigs in Henan province.METHODS:
A total of 623 swine sera, collected from 5 districts, were divided into two groups, under 3-month of age and over 3-month of age. They were tested for HEV antigen and antibody by using ELISAs, respectively. The sera positive for HEV antigen were tested for HEV RNA with RT-PCR. The positive products of RT-PCR were cloned and sequenced.RESULTS:
The positive rates of anti-HEV antibody of the groups under 3-month and over 3-month of age were 90.27% and 92.55%, respectively, without statistical difference, while those of HEV antigen were 15.93% and 5.69%, respectively, with significant difference. The positive rates of anti-HEV antibody and HEV antigen were significantly different among different districts. HEV RNA was detectable in 5 of 47 HEV antigen positive samples. The sequence analysis showed that in 4 of 5 specimens the sequence belonged to genotype 4 while in the remaining one the sequence was genotype 1.CONCLUSION:
The prevalence rate of HEV infection in pigs was high in Henan province and the rate differed in different districts.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Porcinos
/
Enfermedades de los Porcinos
/
Virus de la Hepatitis E
/
Hepatitis E
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Asunto de la revista:
VIROLOGIA
Año:
2008
Tipo del documento:
Article
País de afiliación:
China