A pathway of lysosomal proteolysis mediated by the 73-kilodalton heat shock cognate protein.
Acta Biol Hung
; 42(1-3): 39-47, 1991.
Article
en En
| MEDLINE
| ID: mdl-1844319
ABSTRACT
Cultured IMR-90 diploid human lung fibroblasts respond to withdrawal of serum or growth factors by increasing protein degradation. This increase, due to enhanced transfer of proteins into lysosomes, is specific for a class of intracellular proteins containing peptide sequences biochemically related to Lysine-Phenylalanine-Glutamate-Arginine-Glutamine (KFERQ). This peptide motif is recognized by an intracellular protein which facilitates its transfer into lysosomes in vitro and presumably, in vivo. We called this protein the peptide recognition protein of 73-kilodaltons (prp73). We have shown prp73 to be the constitutive member of the heat shock 70kD family (hsc73) by a variety of criteria. Furthermore, our reconstitution of this pathway of lysosomal degradation in vitro has provided insight in to the molecular mechanisms and requisite biochemical components.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Proteínas de Choque Térmico
/
Lisosomas
Límite:
Humans
Idioma:
En
Revista:
Acta Biol Hung
Año:
1991
Tipo del documento:
Article