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Lipodepsipeptides from Pseudomonas syringae are partially proteolyzed and are not absorbed by humans: an in vitro study.
Fiore, A; Laparra, J M; Farrè, R; Fullone, M R; Grgurina, I; Gallo, M; Fogliano, V.
Afiliación
  • Fiore A; Dipartimento di Scienza degli Alimenti, Università di Napoli Federico II, Parco Gussone, Edificio 84, 80055 Portici, Naples, Italy.
J Food Prot ; 71(5): 979-85, 2008 May.
Article en En | MEDLINE | ID: mdl-18522033
There are some concerns about the use of Pseudomonas-based products as biocontrol agents because of the hemolytic activity shown by their metabolites. The effects of Pseudomonas lipodepsipeptides (LDPs) on mammals via ingestion and the LDP degradation during the digestion and intestinal permeability have not been evaluated. In this research, the susceptibility of different LDPs to degradation was assayed with enzymatic gastrointestinal digestion, and intestinal permeability to LDPs was investigated in an in vitro system based on an intestinal cell layer system. Results demonstrated that trypsin and chymotrypsin hydrolyze up to 50% of the various LDPs, and that proteolysis was further increased by pronase E treatment. A decrease in LDP hemolytic activity matched LDP degradation during the various steps of the digestion process. Moreover, it was shown that syringomycin E (SRE), the main known LDP, was not able to cross the intestinal cell layer, suggesting that SRE does not reach the bloodstream in vivo. It was concluded that the Pseudomonas-based biocontrol products do not represent a serious risk for consumer health. In fact, LDPs possibly present on biocontrol-treated agricultural commodities would likely be partially digested by gastrointestinal enzymes and would not be absorbed at the intestinal level.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Pseudomonas syringae / Digestión / Absorción Intestinal Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: J Food Prot Año: 2008 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Pseudomonas syringae / Digestión / Absorción Intestinal Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: J Food Prot Año: 2008 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos