Interaction between nitroheterocyclic compounds with beta-cyclodextrins: phase solubility and HPLC studies.
J Pharm Biomed Anal
; 47(4-5): 865-9, 2008 Aug 05.
Article
en En
| MEDLINE
| ID: mdl-18534804
ABSTRACT
Chagas disease is a serious health problem for Latin America. Nitrofurazone (NF) and Hidroxymethylnitrofurazone (NFOH) are active against Trypanosoma cruzi. The effect of beta-cyclodextrin (beta-CD) and dimethyl-beta-cyclodextrin (DM-beta-CD) complexation on the UV absorption and retention time of nitrofurazone (NF) and its hydroxymethylated analog (NFOH) were studied in solution. The retention behavior was analyzed on a reversed phase C18 column and the mobile phase used was acetonitrile-water (20/80 v/v), in which cyclodextrins (beta-CD or DM-beta-CD) were incorporated as a mobile phase additive. The decrease in the retention times of NF (or NFOH) with increasing concentration of HP-beta-CD enables the determination of the complex stability constants by HPLC. A phase-solubility study was performed, according to the method reported by Higuchi and Connors, to evaluate the changes of NF/NFOH in the complexation state, and the diagrams obtained suggested that it forms complexes with a stoichiometry of 11. This is an important study for the characterization of potential formulations to be used as therapeutic options for the treatment of Chagas disease.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Beta-Ciclodextrinas
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Excipientes
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Nitrofurazona
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil