Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter.
Biosci Biotechnol Biochem
; 72(6): 1638-41, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18540079
ABSTRACT
We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Expresión Génica
/
Adenoviridae
/
Regiones Promotoras Genéticas
/
Telomerasa
/
Neoplasias
Límite:
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón