Growth factor effects on costal chondrocytes for tissue engineering fibrocartilage.
Cell Tissue Res
; 333(3): 439-47, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18597118
ABSTRACT
Tissue-engineered fibrocartilage could become a feasible option for replacing tissues such as the knee meniscus or temporomandibular joint disc. This study employed five growth factors (insulin-like growth factor-I, transforming growth factor-beta1, epidermal growth factor, platelet-derived growth factor-BB, and basic fibroblast growth factor) in a scaffoldless approach with costal chondrocytes, attempting to improve biochemical and mechanical properties of engineered constructs. Samples were quantitatively assessed for total collagen, glycosaminoglycans, collagen type I, collagen type II, cells, compressive properties, and tensile properties at two time points. Most treated constructs had lower biomechanical and biochemical properties than the controls with no growth factors, suggesting a detrimental effect, but the treatment with insulin-like growth factor-I tended to improve the constructs. Additionally, the 6-week time point was consistently better than that at 3 weeks, with total collagen, glycosaminoglycans, and aggregate modulus doubling during this time. Further optimization of the time in culture and exogenous stimuli will be important in making a more functional replacement tissue.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Costillas
/
Condrocitos
/
Ingeniería de Tejidos
/
Péptidos y Proteínas de Señalización Intercelular
/
Fibrocartílago
/
Matriz Extracelular
Límite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos